Cellife Health Blog, Uncategorized

Who First Discovered NAD+

The History of the ‘Miracle Molecule’, NAD+: The Modern Fountain of Youth

by Cellife Healthcare.

Nowadays, there are many published studies on the benefits of the incredible coenzyme that exists in every cell in the human body: NAD+, commonly known as anti-aging coenzyme 1. The chemical name is nicotinamide adenine dinucleotide.
NAD+ was first discovered by Sir Arthur Harden in 1906. Sir Arthur was studying the fermentation process, that is, the process by which yeast metabolizes sugars and produces alcohol and carbon dioxide. As a result, a substance related to the structure of nucleic acid in the organism was discovered for the first time in the boiled yeast extract. Substances participate in the biochemical reaction process of organisms. Arthur Harden and Hans von Euler-Chelpin became very interested in this mysterious substance. They spent nearly 20 years studying NAD+ and finally discovered the chemical structure of NAD+, thus sharing the 1929 Nobel Prize in Chemistry.

Euler-Chelpin and they found that the structure of NAD+ is composed of two nucleotides, and nucleotides are the basis of the nucleic acids that make up DNA. This finding indicates that fermentation is a metabolic process in which the biochemical process relies on NAD+, which indicates that NAD+ as we know it now plays a key role in human metabolism. [1]
The discoveries of Arthur Harden and Hans von Euler-Chelpin opened a “Pandora’s Box” in the field of human life sciences. After that, many researchers have carried out in-depth research in the fields of biochemical metabolism, catalysis, enzymes, and coenzymes in the human body, providing many valuable results for humans to study the development process of life.
In 1904, the British biochemist Sir Arthur Harden first discovered NAD+.
In 1920, Hans von Euler-Chelpin, winner of the Nobel Prize in Chemistry, isolated and purified NAD+ for the first time and discovered its dinucleotide structure.

In 1930, Nobel Laureate in Physiology or Medicine Otto Heinrich Warburg (Otto Heinrich Warburg) first discovered the key role of NAD+ as a coenzyme in material and energy metabolism.
In 1980, George Birkmyre, a professor in the Department of Medical Chemistry at the University of Graz, Austria, applied reduced NAD+, or NADH, to disease treatment for the first time.
In 2000, Leonard Guarente’s research group found that NAD+-dependent sir2 protein can extend the lifespan of S. cerevisiae, and NAD+-dependent sir2.1 protein can extend the lifespan of Caenorhabditis elegans by nearly 50%.
In 2004, the research group of chemist Stephen L. Helfand (Stephen L. Helfand) found that the NAD+ dependent sir2 (dsir2) protein can extend the lifespan of fruit flies by approximately 10%-20%.
In 2012, Haim Y. Cohen’s research group found that NAD+-dependent SIRT6 protein can prolong the lifespan of male mice by more than 10%.
In 2016, the research groups of Johan Auwerx, Shin-ichiro lmai, Vilhelm A. Bohr and others of the Swiss Federal Institute of Technology Lausanne all discovered the mechanism of NAD+ supplementation to delay aging of human cells. [2]
It can be said that scientists have never stopped the research on the anti-aging coenzyme—NAD+. Because NAD+ artificial synthesis is more difficult. It cannot be supplemented directly, so the researchers set their sights on the precursor nutrients of NAD+. By supplementing NAD+ precursor substances, it helps cells in the body to synthesize NAD+.
Anti-aging coenzyme-precursor nutrient
The currently approved NAD+ precursor nutritional supplement ingredients include the following four types: NR-Nicotinamide Ribose
NA + NAM Niacin and Niacinamide
NMN-Nicotinamide Mononucleotide
NADH-nicotinamide adenine dinucleotide hydride, the reduced state of NAD+, a reduced coenzyme one

  1. NR-nicotinamide ribose is the first NAD+ precursor substance to be discovered. After NR enters the human body, it needs to be phosphorylated by NPK1-2 to be converted to NMN, and then from NMN to NAD+. However, the conversion of NR is more difficult. Most of them are not converted into NMN, but converted into nicotinamide. For supplementing NAD+, the effect of precursor NR is open to question.
  2. Niacin + niacinamide is a relatively economical and convenient way to supplement the precursor of NAD+. The combination of niacin and nicotinamide can synthesize NDA+ in the body with the help of rate-limiting enzymes.
    Cellife Health Care of Australia first developed the NAD+ precursor nutritional supplement Cellife Premium NAD+ in Australia. This product is developed using the combined principle of niacin and niacinamide. And obtained the approval number of the Australian Drug Administration TGA. Cellife Premium NAD+ Australian Drug Administration TGA lot number: AUST L 315381
  3. NMN-nicotinamide mononucleotide. NMN is currently a more effective way to supplement NAD+ precursors. NMN itself is a naturally occurring substance in the human body, and it also exists in many foods, and it is purely natural and harmless.
    NMN can effectively replenish NAD+ in the body. The effect of NMN is better than the combination of niacin + niacinamide. However, NMN only works at high purity. The purity of the active ingredients of Cellife NMN996 and Cellife NMN999 is as high as 99.5%, which can effectively achieve the effect of quickly replenishing NAD+ in the body. Cellife NMN product is the first NMN precursor nutritional supplement product approved by TGA in Australia.
  4. The most effective way to supplement NAD+ is to supplement NADH. NADH is the reduced state of nicotinamide adenine dinucleotide, which is called reduced coenzyme 1, commonly known as mitochondrial.
    Several questions about Cellife NADH
    What is NADH?
    NADH is the abbreviation for nicotinamide adenine dinucleotide hydride, a substance naturally occurring in the human body. “H” stands for high-energy hydrogen, indicating that the substance may be the most biologically active form. NADH is an important coenzyme of the human body, the reduced state of NAD+. It can be converted into NAD+ in the body.
    In addition, NADH is mainly involved in material and energy metabolism in cells. NADH transfers energy to ATP synthesis through oxidative phosphorylation on the inner mitochondrial membrane, so NADH is also called mitochondrial. In theory, the energy released by 1 molecule of NADH can synthesize 2.5 molecules of ATP.
    The content of NADH is directly related to the amount of ATP produced. The more NADH contained in each cell, the more energy it produces. The more energy the organ needs, the higher the amount of NADH contained (or required)
    NADH plays an important role in maintaining cell growth, differentiation, energy metabolism, and cell protection.
    NADH is a control marker in the energy production chain in mitochondria.
    After NADH is converted to NAD+, it is a catalyst for many biological reactions. NADH is essential for cell replication and energy production. NADH is also an important antioxidant. In fact, scientists admit that NADH is the most powerful antioxidant that protects cells from harmful substances.
    An important role of NADH is as a cellular immune response enhancer. NADH and its predecessor, NADPH, contribute to the “killing mechanism” of the immune system against foreign objects. The relationship between NAD+ and the precursor nutrient NADH
    Image source https://www.bioserendipity.com/anti-aging-supplements-ii/nad_nadh/
    NADH also produces good results in high concentrations. The purity of Cellife NADH is as high as 98%, which is the first in Australia and the only NADH approved by TGA in Australia.
    The main functions of Cellife NADH:
    • Help cells take up vitamins • Help cells take up energy
    • Enhance immune system function

• Anti-oxidation Reduce free radicals • Help improve metabolism • Promote tissue cell regeneration • Delay cell aging
Cellife NADH is approved by the Australian Drug Administration TGA and is safe and effective.
What is the best time to supplement Cellife NADH?
The best time to take Cellife NADH is in the morning, 30 minutes before breakfast. Generally take one tablet if you need more energy in the afternoon or evening, you can also take another tablet.
How long do I need to take it at first?
For normal healthy people, taking it for one month continuously can feel that the body’s energy is improved and the health status will also be improved. It is generally recommended to take Cellife NADH continuously for at least one month. Generally speaking, after 8 weeks of continuous use of Cellife NADH, you can feel a relatively significant improvement in your physical condition.
Can I take it before/during pregnancy?
Every pregnant woman’s physical condition is different, so it is best to consult your doctor. However, in theory, NADH naturally exists in the body and is a substance that exists in our body itself, so it will not cause harm to mothers or babies in theory.
How to store Cellife NADH?
For best results, we recommend storing Cellife NADH tablets in a dry and cool place, because NADH will be destroyed by heat and moisture.
How long does it take to have an effect after taking Cellife NADH?
The answer to this question varies from person to person. Each of us has our own biochemical reaction efficiency: our bodies absorb food and nutrients at different rates. Some people can feel the effect the same day after taking it. For example, with a clear brain and improved physical energy, if you climb the stairs, you will find that climbing the stairs becomes easier than usual. However, most healthy people report that they can feel an improvement in physical energy within five to ten days of taking Cellife NADH. After all, NADH must fully enter the billions of cell structures in the body and participate in the body’s biochemical metabolism before it can begin to produce effects. Therefore, first-time users may need to wait patiently to see beneficial results.
Are there any side effects from taking Cellife NADH?
Because NADH is also a substance in the human body, taking Cellife NADH will not cause any obvious side effects. However, everyone has different allergens and allergic reactions. So especially for people with allergies, the best way is to consult a doctor first.
Can I take it with other medicines/supplements?

There are currently no drugs that adversely affect NADH. During the period of taking some drugs, you can take Cellife NADH. For example, the most commonly used antihypertensive drugs, antidepressants and antihistamines. However, if you are taking medication prescribed by your doctor, or if you are not sure about your physical health, please consult your healthcare professional first.
[1] Zhihu “What exactly is NAD+ that makes people live to 150 years old? Why is it so important?” [2] “NAD+, an anti-aging topic popular with Noel Prize winners”

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